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Contents 1 PCR and digestion controls 1.1 Aims 1.2 ... 1.3 Results 1.4 Discussion 2 yneA

PCR and digestion controls

Aims

Since we have recently had problems with both PCR and restriction digests, we set up positive controls to ensure that there is nothing wrong with any of our enzymes or ...

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Results

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Discussion

Worked correctly.. nothing is wrong with our enzymes.. in the case of our rocF plasmid PCRs, it must be the primers which are the problem.. new ones are supposed to be arriving today.. in the other cases, repeat..

yneA