Supplemented medium

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  • 50 ml 1X diluted Spizizen's salts
  • 0.5 ml(0.02%) casamino acids (2%)
  • 0.5 ml(14 µg/ml) tryptophane (100X)
  • 1.25 ml (0.5%)glucose (20%)
  • 50 µl (22 µg/ml) FeNH4 citrate (1000X) (prepare fresh every 2 weeks! )
  • antibiotics
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