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20:59, 27 October 2010 (diff | hist) SMG17MC ‎ (top)
20:58, 27 October 2010 (diff | hist) N GSM17- Agar ‎ (New page: '''GSM17- Agar''' - M17-Agar - 0.5 M Sucrose - 0.5% Glucose) (top)
20:56, 27 October 2010 (diff | hist) N SMG17MC ‎ (New page: '''SMG17MC''' -M17 -o.5 M Sucrose -0.5 % Glucose -20 mM MgCl2 -2 mM CaCl2)
20:54, 27 October 2010 (diff | hist) Team:Groningen/Protocols for Lactococcus ‎ (top)
20:53, 27 October 2010 (diff | hist) N Wash buffer ‎ (New page: '''Wash buffer''' -0.5 M Sucrose (17g/100ml) -10% gylcerol( 11.5ml/100 ml)) (top)
20:51, 27 October 2010 (diff | hist) N SMGG ‎ (New page: '''SMGG''' -M17 -0.5 M Sucrose -0.5% Glucose -1% Glycine) (top)
20:49, 27 October 2010 (diff | hist) N Team:Groningen/Protocols for Lactococcus ‎ (New page: '''Preparation of cells''' *10 ml ON culture (SMGG) in 100 ml SMGG *Grow to OD600=0.2-0.7 *Wash three times with 50 ml icecold wash buffer*Resuspend in 1 ml wash buffer '''Electr...)
19:56, 27 October 2010 (diff | hist) Team:Groningen/Protocols ‎ (→Protocols)
16:19, 25 October 2010 (diff | hist) Team:Groningen/Protocols for Streptomyces ‎ (top)
15:44, 25 October 2010 (diff | hist) N Team:Groningen/Antibiotics and Concentrationas ‎ (New page: '''Antibiotics''' Ampicillin 100 mg/ml Ampicillin (1000x) Stock *1 g of Ampicillin sodium salt in 10 mL of demiwater (or 50% EtOH) *Add NaOH or KOH to allow the Ampicillin to dissol...) (top)
15:44, 25 October 2010 (diff | hist) Team:Groningen/Protocols ‎ (→Protocols)
15:42, 25 October 2010 (diff | hist) m Team:Groningen/Antibiotics Concentrationas and Organisms ‎ (top)
15:23, 25 October 2010 (diff | hist) Team:Groningen/Antibiotics Concentrationas and Organisms ‎
17:22, 9 September 2010 (diff | hist) Team:Groningen/Normal SDS-PAGE using Tris-Glycine Gels and Electrophoresis Buffer ‎ (top)
17:22, 9 September 2010 (diff | hist) N Team:Groningen/Protocols for Bacillus subtilis 168 ‎ (New page: '''2-step transformation procedure for ''Bacillus subtilis'' 168''' *Cells are grown O/N at 37 °C in supplemented medium contains Spizizen's salts (without antibiotics) *Dilute c...) (top)
17:20, 9 September 2010 (diff | hist) Team:Groningen/Protocols ‎ (→Protocols)
17:20, 9 September 2010 (diff | hist) Team:Groningen/Protocols ‎ (→Protocols)
17:19, 9 September 2010 (diff | hist) Team:Groningen/Growing Streptomyces ‎ (top)
17:19, 9 September 2010 (diff | hist) N Team:Groningen/Antibiotics Concentrationas and Organisms ‎ (New page: '''Concentrations for antibiotics ''' '''Antibiotic''' ''' Stock''' ''' E.coli ''' ''' B.subtilis''' *Ampicillin (Amp) (in H2O) 50 mg/ml (4 °C...)
17:15, 9 September 2010 (diff | hist) Team:Groningen/Protocols ‎ (→Protocols)
17:13, 9 September 2010 (diff | hist) N Team:Groningen/Protocols for Streptomyces ‎ (New page: '''Growth condition''' -Make plates with a thick layer of medium, 100 ml medium per big Petri dish.Medium of Streptomyces is mannitol soya flour medium (MS or SFM) 300 ml per 1L bottle to...)
17:11, 9 September 2010 (diff | hist) Team:Groningen/Protocols ‎ (→Protocols)
17:09, 9 September 2010 (diff | hist) Team:Groningen/Growing Streptomyces ‎
17:04, 9 September 2010 (diff | hist) Team:Groningen/Growing Streptomyces ‎
17:02, 9 September 2010 (diff | hist) Team:Groningen/Chaplins ‎ (top)
14:59, 9 September 2010 (diff | hist) N Team:Groningen/Chaplins ‎ (New page: '''Expression Chaplins in NZ8900''' -Grow O/N culture in Ty with antibiotics (Km5 and Cm5) -Measure OD600 (with 0.9 ml with 100 μL of O/N culture, number *10=OD) -Inoculate 15ml TY (wi...)
14:57, 9 September 2010 (diff | hist) Team:Groningen/Protocols ‎ (→Protocols)
14:55, 9 September 2010 (diff | hist) Team:Groningen/Protocols ‎ (→Protocols)
14:51, 9 September 2010 (diff | hist) N Procedures for Bacillus subtilis 168 ‎ (New page: '''2-step transformation procedure for ''Bacillus subtilis'' 168''' *Cells are grown O/N at 37 °C in supplemented medium contains Spizizen's salts (without antibiotics) *Dilute ce...) (top)
14:49, 9 September 2010 (diff | hist) Team:Groningen/Protocols ‎ (→Protocols)
18:07, 31 August 2010 (diff | hist) Team:Groningen/Growing Streptomyces ‎
17:37, 31 August 2010 (diff | hist) Team:Groningen/Growing Streptomyces ‎
16:47, 31 August 2010 (diff | hist) Team:Groningen/Growing Streptomyces ‎
19:11, 28 August 2010 (diff | hist) Team:Groningen/Growing Streptomyces ‎
17:54, 28 August 2010 (diff | hist) N Team:Groningen/2-step Transformation Procedure for Bacillus subtilis 168 ‎ (New page: '''2-step transformation procedure for ''Bacillus subtilis'' 168''' *Cells are grown O/N at 37 °C in supplemented medium contains Spizizen's salts (without antibiotics) *Dilute ce...) (top)
17:54, 28 August 2010 (diff | hist) Team:Groningen/Protocols ‎ (→Protocols)
17:53, 28 August 2010 (diff | hist) Team:Groningen/Protocols ‎ (→Protocols)
23:48, 24 August 2010 (diff | hist) Team:Groningen/Growing Streptomyces ‎
23:02, 24 August 2010 (diff | hist) Team:Groningen/Growing Streptomyces ‎
22:44, 24 August 2010 (diff | hist) Team:Groningen/CaCl2 Competent Cells ‎ (top)
22:32, 24 August 2010 (diff | hist) N TY medium ‎ (New page: '''Ingredients''' -10 g Bacto-tryptone -5 g yeast extract -10 g NaCl '''Protocol''' -Mix dry ingredients and add distilled water up to 1 Liet -Pour into 2 L flask (or greater) -Auto...) (top)
22:30, 24 August 2010 (diff | hist) N Starvation medium ‎ (New page: Starvation medium: 50 ml 1X Spizizen's salts+ 0.5% glucose (better not to add antibiotics)) (top)
22:28, 24 August 2010 (diff | hist) N Supplemented medium ‎ (New page: *50 ml 1X diluted '''Spizizen's salts''' *0.5 ml(0.02%) '''casamino acids''' (2%) *0.5 ml(14 µg/ml) '''tryptophane''' (100X) *1.25 ml (0.5%)'''glucose''' (20%) *50 µl (22 µg/ml) '''FeN...) (top)
20:12, 24 August 2010 (diff | hist) N Spizizen's salts ‎ (New page: Spizizen's 5X diluted salts solution contains of (per liter): *K2HPO4, 14.8 g/l (85 mM) *KH2PO4, 5.4 g/l (40 mM) *(NH4)2SO4, ...) (top)
20:03, 24 August 2010 (diff | hist) Team:Groningen/Normal SDS-PAGE using Tris-Glycine Gels and Electrophoresis Buffer ‎
19:46, 24 August 2010 (diff | hist) Team:Groningen/Protocols ‎ (→Protocols)
19:44, 24 August 2010 (diff | hist) Team:Groningen/Protocols ‎
13:58, 24 August 2010 (diff | hist) Normal SDS-PAGE using Tris-Glycine Gels and Electrophoresis Buffer ‎
13:48, 24 August 2010 (diff | hist) Normal SDS-PAGE using Tris-Glycine Gels and Electrophoresis Buffer ‎
13:26, 24 August 2010 (diff | hist) N Normal SDS-PAGE using Tris-Glycine Gels and Electrophoresis Buffer ‎ (New page: '''Wear gloves at all times!''' Requirements: *30% acrylamide(Biorad) '''NEUROTOXIN!''' *4X separation gel buffer: 1.5M Tris.HCl, 0.4% SDS pH6.8 *4X stacking gel buffer: 0.5M Tris.HCl, 1...)

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