Team:Edinburgh/Bacterial/Blue light producer

• home
• team - illuminati
  • official
  • supervisors
  • advisors
  • students
  • environment
  • gallery
• genomic BRIDGEs
  • the protocol
  • submitted parts
  • results
  • future work
  • references
• bacterial BRIDGEs
  • the repressilator
  • red light
  • red sensor
  • blue light
  • blue sensor
  • green light
  • green sensor
  • submitted parts
  • results
  • future work
  • references
• modelling BRIDGEs
  • kappa
  • the genomic model
  • the bacterial model
  • the signalling model
  • results
  • future work
  • references
• human BRIDGEs
  • human aspects
  • results
  • future work
  • references
• lab notes   
  • collaboration
  • BRIDGE
  • red light
  • red sensor
  • blue light
  • blue sensor
  • green light
  • green sensor
  • safety
  • protocols
• acknowledgements

Overview: The blue light producer

The construction of a blue light producer will require the combination of luxAB with an extra protein called lumazine (lumP). This will shift the wavelength of the bacterial luciferase towards the blue spectrum, giving us a light which can activate our blue light sensor. Last year's Edinburgh team already made both of these parts so we are hoping to be able to test and characterise them soon.

Problems

The luxAB which last year's team made and stored is failing to transform correctly. Colonies that ought to be growing white are growing blue (luxAB should have replaced the lacZ' in the vector, EdinbrickI). A similar thing is happening with lumP and RFP, where cells are still growing red, despite the fact that the RFP ought to have been removed when lumP was inserted last year. We have come across several PCR products not yet inserted into vectors with which we are hoping to start this part of the project (i.e. the bit which should already have been done by last year according to their lab notes) again.

Biobricks

Both lumP and luxAB have already been submitted as biobricks in pSB1A3, but if they give everyone else as much trouble as they've given us, we may have to resubmit them.

References