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Team:Cambridge/Biobrickingluxoperon
From 2010.igem.org
Contents |
lux oligos
luxI
forward
gaattcgcggccgcttctagagAAGGGAGGTTGGTATGACTAT
dG=0.11 Tm=58.45
reverse coding
atgatcatcgccggcgacgtcTTAATTTAAGACTGCTTTTTTAAACT
dG=-0.34 Tm=57.3
reverse RBS
atgatcatcgccggcgacgtcATAATATACTTAGTATTTAAAATAAATTAATGATTTA
dG=-0.34 Tm=56.83
luxC
forward
gaattcgcggccgcttctagagAGGGAAAATAATGAATAAATGTAT
Tm=56.07 dG=0.76
reverse coding
atgatcatcgccggcgacgtcCTATGGGACAAAAACTAAAAAC
dG=-0.77 Tm=56.58
reverse RBS
atgatcatcgccggcgacgtcTAACTATGGGACAAAAACTAAA
dG=-0.34 Tm=55.31
luxD
forward
gaattcgcggccgcttctagagAAGGAAATTATATGAAAGATGAAA
Tm=57.08 dG=0.11
reverse coding
atgatcatcgccggcgacgtcTTAAGCCAATTCTAATAATTCATT
dG=-0.34 Tm=57.19
reverse RBS
atgatcatcgccggcgacgtcTTGGTGATTCTGTTTATTTAAG
dG= -0.34 Tm=55.69
luxA
forward
gaattcgcggccgcttctagagAAAGGAATAGAGTATGAAGTTTGG
Tm=58.8 dG=0.76
reverse coding
atgatcatcgccggcgacgtcTTATTTAGGTTCTTTTAAGAAAGGAG
dG=-0.34 Tm=58.66
reverse RBS
atgatcatcgccggcgacgtcTTATCTCTAGTATCAAATAAGTAATTTATTTAGG
dG=-0.34 Tm=58.84
luxB
forward
gaattcgcggccgcttctagagTAAGGAACAAGTTATGAAATTTG
Tm=57.09 dG=0.47
reverse coding
atgatcatcgccggcgacgtcTTATGGTAAATTCATTTCGATT
dG=-0.34 Tm=57.2
reverse RBS
atgatcatcgccggcgacgtcAATACCTAATATCAACAGAAATTTAAA
dG=-0.34 Tm=56.89
luxE
forward
gaattcgcggccgcttctagagACTGGAGAGGGTATGACTGT
Tm=58.15 dG=0.76
reverse coding
atgatcatcgccggcgacgtcTTAATCCTTGATATTCTTTTGTATG
dG=-0.34 Tm=57.5
reverse RBS
atgatcatcgccggcgacgtcAACTTAATCCTTGATATTCTTTTGTAT
dG=-0.34 Tm=57.96
luxG
forward
gaattcgcggccgcttctagagAAGGATTAAGTTATGATTGTTGA
Tm=56.52 dG=0.11
reverse coding
atgatcatcgccggcgacgtcTTATACGTATGCAAAAGCATC
dG=-1.21 Tm=57.48
reverse RBS
atgatcatcgccggcgacgtcAAAGCCGGATTAATAATCTG
dG=-3.57 Tm=57.82
alternative ===reverse RBS===
atgatcatcgccggcgacgtcTTTATATTCTCTTTATACGTATGCAA
dG=-0.34 Tm=57.36
