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From 2010.igem.org

Contents 1 lux oligos 1.1 luxI 1.1.1 forward 1.1.2 reverse coding 1.1.3 reverse RBS 1.2 luxC 1.2.1 forward 1.2.2 reverse coding 1.2.3 reverse RBS 1.3 luxD 1.3.1 forward 1.3.2 reverse coding 1.3.3 reverse RBS 1.4 luxA 1.4.1 forward 1.4.2 reverse coding 1.4.3 reverse RBS 1.5 luxB 1.5.1 forward 1.5.2 reverse coding 1.5.3 reverse RBS 1.6 luxE 1.6.1 forward 1.6.2 reverse coding 1.6.3 reverse RBS 1.7 luxG 1.7.1 forward 1.7.2 reverse coding 1.7.3 reverse RBS

lux oligos

luxI

forward

gaattcgcggccgcttctagagAAGGGAGGTTGGTATGACTAT 

dG=0.11 Tm=58.45

reverse coding

atgatcatcgccggcgacgtcTTAATTTAAGACTGCTTTTTTAAACT

dG=-0.34 Tm=57.3

reverse RBS

atgatcatcgccggcgacgtcATAATATACTTAGTATTTAAAATAAATTAATGATTTA

dG=-0.34 Tm=56.83

luxC

forward

gaattcgcggccgcttctagagAGGGAAAATAATGAATAAATGTAT

Tm=56.07 dG=0.76

reverse coding

atgatcatcgccggcgacgtcCTATGGGACAAAAACTAAAAAC

dG=-0.77 Tm=56.58

reverse RBS

atgatcatcgccggcgacgtcTAACTATGGGACAAAAACTAAA

dG=-0.34 Tm=55.31

luxD

forward

gaattcgcggccgcttctagagAAGGAAATTATATGAAAGATGAAA

Tm=57.08 dG=0.11

reverse coding

atgatcatcgccggcgacgtcTTAAGCCAATTCTAATAATTCATT

dG=-0.34 Tm=57.19

reverse RBS

atgatcatcgccggcgacgtcTTGGTGATTCTGTTTATTTAAG

dG= -0.34 Tm=55.69

luxA

forward

gaattcgcggccgcttctagagAAAGGAATAGAGTATGAAGTTTGG

Tm=58.8 dG=0.76

reverse coding

atgatcatcgccggcgacgtcTTATTTAGGTTCTTTTAAGAAAGGAG

dG=-0.34 Tm=58.66

reverse RBS

atgatcatcgccggcgacgtcTTATCTCTAGTATCAAATAAGTAATTTATTTAGG

dG=-0.34 Tm=58.84

luxB

forward

gaattcgcggccgcttctagagTAAGGAACAAGTTATGAAATTTG

Tm=57.09 dG=0.47

reverse coding

atgatcatcgccggcgacgtcTTATGGTAAATTCATTTCGATT

dG=-0.34 Tm=57.2

reverse RBS

atgatcatcgccggcgacgtcAATACCTAATATCAACAGAAATTTAAA

dG=-0.34 Tm=56.89

luxE

forward

gaattcgcggccgcttctagagACTGGAGAGGGTATGACTGT

Tm=58.15 dG=0.76

reverse coding

atgatcatcgccggcgacgtcTTAATCCTTGATATTCTTTTGTATG

dG=-0.34 Tm=57.5

reverse RBS

atgatcatcgccggcgacgtcAACTTAATCCTTGATATTCTTTTGTAT

dG=-0.34 Tm=57.96

luxG

forward

gaattcgcggccgcttctagagAAGGATTAAGTTATGATTGTTGA

Tm=56.52 dG=0.11

reverse coding

atgatcatcgccggcgacgtcTTATACGTATGCAAAAGCATC

dG=-1.21 Tm=57.48

reverse RBS

atgatcatcgccggcgacgtcAAAGCCGGATTAATAATCTG

dG=-3.57 Tm=57.82

alternative ===reverse RBS===

atgatcatcgccggcgacgtcTTTATATTCTCTTTATACGTATGCAA

dG=-0.34 Tm=57.36