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From 2010.igem.org

Phosphoreum Oligos

ribA Reverse Primer:

Sequence: tactagtagcggccgctgcagAAATGAACGTTAGTTCCAAC

Structure: one hairpin in suffix/coding region

Tm: 56.27 deg

dG: 0.48 kcal/mol

luxE Reverse Primer:

Sequence: tactagtagcggccgctgcagTATGATCTATTTTAAACCTGATGA

Structure: one hairpin in suffix

Tm: 56.59 deg

dG: 0.57 kcal/mol

Pbad Reverse C-end:

Sequence: tctttatcatacaatctccttaGCTAGCCCAAAAAAAC

Structure: one small hairpin in gene

Tm: 53.68 deg

dG: 2.30 kcal/mol

Lux C forward primer:

Sequence: AGGAGATTGTATGATAAAGAAAAT

Tm: 56.26

dG: 1.98

lux A(XbaI) forward:

Sequence: TACTGGACActagaGCATCATT

Tm: 58.46

dG: 1.53

lux A(XbaI) reverse:

Sequence: AATGATGCtctagTGTCCAGTA

Tm: 58.46

dG: 2.53

lux E(SpeI) Forward:

Sequence: GTAGTGGTacCagtGGTGTAAA

Tm: 58.54

dG: 1.41

lux E(SpeI) reverse:

Sequence: TTTACACCactGgtACCACTAC

Tm: 58.54

dG: 1.25

lux G(XbaI) forward:

Sequence: TGCCTGTCctagaAAACTTT

Tm: 58.56

dG: 1.77

luxG(XbaI) reverse:

Sequence: AAAGTTTtctagGACAGGCA

Tm: 58.56

dG: 1.33

ribB (EcoRI i) forward:

Sequence: GAATATGGTgaGttcAATATGATC

Tm: 58.39

dG: 1.91

ribB (EcoRI i) reverse:

Sequence: GATCATATTgaaCtcACCATATTC

Tm: 58.39

dG: 1.91

ribB (EcoRI ii) forward:

Sequence: TGAGCCgaaCtcTCAAAC

Tm: 59.64

dG: 1.26

ribB (EcoRI ii) reverse:

Sequence: GTTTGAgaGttcGGCTCA

Tm: 59.64

dG: 1.16