Team:Cambridge/PhosphoreumOligos
From 2010.igem.org
Phosphoreum Oligos
ribA Reverse Primer:
- Sequence: tactagtagcggccgctgcagAAATGAACGTTAGTTCCAAC
- Structure: one hairpin in suffix/coding region
- Tm: 56.27 deg
- dG: 0.48 kcal/mol
luxE Reverse Primer:
- Sequence: tactagtagcggccgctgcagTATGATCTATTTTAAACCTGATGA
- Structure: one hairpin in suffix
- Tm: 56.59 deg
- dG: 0.57 kcal/mol
Pbad Reverse C-end:
- Sequence: tctttatcatacaatctccttaGCTAGCCCAAAAAAAC
- Structure: one small hairpin in gene
- Tm: 53.68 deg
- dG: 2.30 kcal/mol
Lux C forward primer:
- Sequence: AGGAGATTGTATGATAAAGAAAAT
- Tm: 56.26
- dG: 1.98
lux A(XbaI) forward:
- Sequence: TACTGGACActagaGCATCATT
- Tm: 58.46
- dG: 1.53
lux A(XbaI) reverse:
- Sequence: AATGATGCtctagTGTCCAGTA
- Tm: 58.46
- dG: 2.53
lux E(SpeI) Forward:
- Sequence: GTAGTGGTacCagtGGTGTAAA
- Tm: 58.54
- dG: 1.41
lux E(SpeI) reverse:
- Sequence: TTTACACCactGgtACCACTAC
- Tm: 58.54
- dG: 1.25
lux G(XbaI) forward:
- Sequence: TGCCTGTCctagaAAACTTT
- Tm: 58.56
- dG: 1.77
luxG(XbaI) reverse:
- Sequence: AAAGTTTtctagGACAGGCA
- Tm: 58.56
- dG: 1.33
ribB (EcoRI i) forward:
- Sequence: GAATATGGTgaGttcAATATGATC
- Tm: 58.39
- dG: 1.91
ribB (EcoRI i) reverse:
- Sequence: GATCATATTgaaCtcACCATATTC
- Tm: 58.39
- dG: 1.91
ribB (EcoRI ii) forward:
- Sequence: TGAGCCgaaCtcTCAAAC
- Tm: 59.64
- dG: 1.26
ribB (EcoRI ii) reverse:
- Sequence: GTTTGAgaGttcGGCTCA
- Tm: 59.64
- dG: 1.16