Team:Cambridge/PhosphoreumOligos

From 2010.igem.org

Phosphoreum Oligos

ribA Reverse Primer:

Sequence: tactagtagcggccgctgcagAAATGAACGTTAGTTCCAAC
Structure: one hairpin in suffix/coding region
Tm: 56.27 deg
dG: 0.48 kcal/mol

luxE Reverse Primer:

Sequence: tactagtagcggccgctgcagTATGATCTATTTTAAACCTGATGA
Structure: one hairpin in suffix
Tm: 56.59 deg
dG: 0.57 kcal/mol

Pbad Reverse C-end:

Sequence: tctttatcatacaatctccttaGCTAGCCCAAAAAAAC
Structure: one small hairpin in gene
Tm: 53.68 deg
dG: 2.30 kcal/mol

Lux C forward primer:

Sequence: AGGAGATTGTATGATAAAGAAAAT
Tm: 56.26
dG: 1.98


lux A(XbaI) forward:

Sequence: TACTGGACActagaGCATCATT
Tm: 58.46
dG: 1.53

lux A(XbaI) reverse:

Sequence: AATGATGCtctagTGTCCAGTA
Tm: 58.46
dG: 2.53


lux E(SpeI) Forward:

Sequence: GTAGTGGTacCagtGGTGTAAA
Tm: 58.54
dG: 1.41

lux E(SpeI) reverse:

Sequence: TTTACACCactGgtACCACTAC
Tm: 58.54
dG: 1.25


lux G(XbaI) forward:

Sequence: TGCCTGTCctagaAAACTTT
Tm: 58.56
dG: 1.77

luxG(XbaI) reverse:

Sequence: AAAGTTTtctagGACAGGCA
Tm: 58.56
dG: 1.33


ribB (EcoRI i) forward:

Sequence: GAATATGGTgaGttcAATATGATC
Tm: 58.39
dG: 1.91

ribB (EcoRI i) reverse:

Sequence: GATCATATTgaaCtcACCATATTC
Tm: 58.39
dG: 1.91


ribB (EcoRI ii) forward:

Sequence: TGAGCCgaaCtcTCAAAC
Tm: 59.64
dG: 1.26

ribB (EcoRI ii) reverse:

Sequence: GTTTGAgaGttcGGCTCA
Tm: 59.64
dG: 1.16