Team:LMU-Munich/Notebook/Apoptosis

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Apoptosis Notebook

Contents

Week Days
31 8-02-2010 8-03-2010 8-04-2010 8-05-2010 8-06-2010 8-07-2010 8-08-2010
32 8-09-2010 8-10-2010 8-11-2010 8-12-2010 8-13-2010 8-14-2010 8-15-2010
33 8-16-2010 8-17-2010 8-18-2010 8-19-2010 8-20-2010 8-21-2010 8-22-2010

8-02-2010

  • Some test text here.

8-03-2010

Some test text in bold We created following tests:

  • test1
  • test2
  • test3

8-04-2010

Example of a table

header 1 header 2 header 3
row 1, cell 1 row 1, cell 2 row 1, cell 3
row 2, cell 1 row 2, cell 2 row 2, cell 3

8-05-2010

text

8-06-2010

text

8-07-2010

text

8-08-2010

text

8-09-2010

text

8-10-2010

Transforming competent cells

- eGFP Biobrick: BBa_I714891 SDY_eGFP (Kanamycin)

- TEV recogn N Degron SF3 = pDS7 (Ampicillin)

- TEV p14 recogn = 190-6 (Ampicillin)

-> Protocol: https://www.openwetware.org/wiki/Transforming_chemically_competent_cells_protocol

- We added 2 µl DNA

- We plated out 200 µl


Plasmid Isolation

- CMV-Promoter Biobrick: BBa_J52034

-> Protocol: http://www.promega.com/protcards/9fb093/fb093.pdf

- Prepared overnight culture, measured concentration of DNA

-> Poor results -> thrown away

8-11-2010

text

8-12-2010

text

8-13-2010

text

8-14-2010

text

8-15-2010

text

8-16-2010

text

8-17-2010

text

8-18-2010

text

8-19-2010

text

8-20-2010

text

8-21-2010

text

8-22-2010

text

 

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