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Contents 1 Transformation of pVeg and lacI 1.1 Results/Aims 1.2 Discussion 2 Transformation of lacI 2.1 Aims 2.2 Materials and Protocol 2.3 Result

Transformation of pVeg and lacI

Results/Aims

• There are colonies on all 3 plates for pVeg.
• For lacI, there is only one colony on one of the normal plates and more on the concentrated one.

Discussion

• For pVeg, we will set up an overnight broth culture from the colonies we got on the plates for plasmid extraction
• To show that we have sucessfully transformed from lacI, we will select 4 colonies and plate them on a section of an agar plate as well as setting up overnight broth cultures.

Transformation of lacI

Aims

To transform some competent E.coli DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on 06.08.10.

Materials and Protocol

Please refer to Transformation of E.coli.

Result

Please refer to 10.08.10 for result.