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Transformation of Bacillus subtilis

Materials required

1. Bacillus subtilis 168
2. 10 µl of DNA
3. SMM medium (1 liter)
   • 2.0 g of ammonium sulphate
   • 14.0g of dipotassium hydrogen phosphate
   • 6.0g of potassium dihydrogen phosphate
   • 1.0g of sodium citrate dehydrate
   • 0.2g of magnesium sulphate
   • Top up the rest of the medium with water

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