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Contents 1 Cloning the rocF BioBrick 1.1 Aim 1.2 Materials and Protocol 1.2.1 PCR 1.3 Gibson assembly

Cloning the rocF BioBrick

Aim

The aim of today's experiment is to amplify 6 different fragments for the construction of rocF BioBrick with the help of 6 different Phusion PCR.

Materials and Protocol

Please refer to PCR for Phusion PCR protocol. The details for the 6 PCR reactions are mentioned below:

PCR

Gibson assembly