"

From 2010.igem.org

Alkane Sensing, Solvent Tolerance and Salt Tolerance

by Pieter

We found it remarkable that the only positive samples we found so far were from the series without preamplifying the insert by PCR (e.g. the AlkS). This is why we decided to retry the insertion of bbc1, PhPFDalpha and PhPFDbeta in J61101, but this time without PCRing the insert.

Digestion

Ligation

Transformation

Emulsifier

While waiting for the sequencing results of BBa_K398202 we already proceed with the insertion of OprG and AlnA.