Friday July 30, 2010

Chris's first gel of the attempted gradient PCR to remove the CpxP promoter from the E. coli genome. This line of tubes ran from 50°C to 56°C and at a MgCl₂ concentration of 1.5 µM.

Chris's second gel of the attempted gradient PCR to remove the CpxP promoter from the E. coli genome. This line of tubes ran from 50°C to 56°C and at a MgCl₂ concentration of 2.0 µM.

Himika

Today there was a meeting which updated updates all the team members about each other. I also kept looking into MATLAB tutorials and tried out some of the tutorials. I also read some of the papers about inclusion bodies. This will be used to model of the project. The sequence data of the I0500-B0034 came back today and it was successful it seems. So I have decided to go forth with the construct and add on more parts next week.