Chris's first gel of the attempted gradient PCR to remove the CpxP promoter from the E. coli genome. This line of tubes ran from 50°C to 56°C and at a MgCl2 concentration of 1.5 µM.
Chris's second gel of the attempted gradient PCR to remove the CpxP promoter from the E. coli genome. This line of tubes ran from 50°C to 56°C and at a MgCl2 concentration of 2.0 µM.