Team:Harvard/color/promoters

From 2010.igem.org

Revision as of 14:41, 30 July 2010 by Jyquinn (Talk | contribs)



targeting the petals

In addition to using a constitutive promoter to express our knockdown constructs, we will also express these constructs in parallel with petal specific promoters. Using Genevestigator's Biomarker Search tool, we identified genes with high petal expression. We then looked up those genes AraGenNet for more specific information about expression, and from those that looked the most promising, found promoter information on the Plant Promoter Database by Yoshiharu Y Yamamoto and Junichi Obokata at the Center for Gene Research at Nagoya University. From this database, we selected sequences from 1 to 1.5 kb upstream from the genes to PCR amplify from arabidopsis genomic DNA.

the genes