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Team:Newcastle/Gel electrophoresis
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Revision as of 09:15, 30 July 2010 by Shethharsh08 (Talk | contribs)
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Gel electrophoresis
Materials
- 1X TAE buffer
- SafeView
- Agarose
- Eppendorf
- Gel making tank
- Gel running tank
Procedures
- Make up 1% agarose gel (1 g of agarose in 100 ml of TAE buffer) and transfer 60 ml of molten agarose gel into the gel tray with 3 μl of Safeview dye.
- Wait for 30 min to allow the gel to harden.
- Transfer harden gel into the gel tank and add TAE buffer until the gel is completely submerge.
- Depending on the nature of the sample, 3μl of GeneRuler™ 1kb Plus DNA Ladder was used for analysing DNA.
- Loading buffer was then added together with the sample before loading onto the gel matrix.
- Run gel at 90V until separation is achieved and visualize using the gelDoc.
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