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Growing an Overnight Culture

Materials Required

• LB Broth
• Colonies of bacteria
• Flame loop
• Universal tube
• Pipette

Procedures

1. 5ml of LB broth is pipetted into the universal tube.
2. The flame loop is used to pick a colony.
3. The colony is then mixed into the LB Broth in the universal tube.
4. The culture is left overnight at 37°C (E.coli, B.subtilis lower temp)

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