Making Media

Revision as of 19:58, 29 July 2010 by Erik (Talk | contribs)

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LB Media (RT)

  * 10g Tryptone
  * 10g NaCl
  * 5g Yeast Extract
  * 1L Deionized Water

SOB Media (RT)

  * 20g Tryptone 
  * 5g Yeast Extract
  * 0.5g NaCl
  * 0.2g KCl
  * 1L Deionized Water

SOC Media (4 degrees Celsius)

  * Add the following per ml SOB:
      - 4ul MgCl2 Solution (100)
      - 8ul Glucose Solution (200)

Glucose Stock Solution (4 degrees Celsius)

  * 5g glucose
  * 10ml Deionized Water