Making Media
From 2010.igem.org
LB Media (RT)
* 10g Tryptone * 10g NaCl * 5g Yeast Extract * 1L Deionized Water
SOB Media (RT)
* 20g Tryptone * 5g Yeast Extract * 0.5g NaCl * 0.2g KCl * 1L Deionized Water
SOC Media (4 degrees Celsius)
* Add the following per ml SOB: - 4ul MgCl2 Solution (100) - 8ul Glucose Solution (200)
Glucose Stock Solution (4 degrees Celsius)
* 5g glucose * 10ml Deionized Water