Team:Harvard/color/notebook
From 2010.igem.org
![](https://static.igem.org/mediawiki/2010/9/93/Harvard2010colorbanner.png)
notebook
Week 6 | - | - | - | - | 07-23-2010 |
Week 7 | 07-26-2010 | 07-27-2010 | 07-28-2010 | 07-29-2010 | 07-30-2010 |
07-23-2010 [top]
- Received primers for LUT2 and BETA-OHASE 1 - began building knockdown constructs
- PCR reaction on RS300 to insert recognition sites. Primers listed below
- LUT2 rxn 1: A, CP4
- LUT2 rxn 2: CP2, CP3
- LUT2 rxn 3: CP1, B
- BETA-OHASE 1 rxn 1: A, CP8
- BETA-OHASE 1 rxn 2: CP6, CP7
- BETA-OHASE 1 rxn 3: CP5, B
- PCR purified completed reaction
07-26-2010 [top]
Bands appeared to be the correct size, so started PCR stitching process
Ran two PCR reactions, one for each of LUT2 and BETA-OHASE 1
Template: Rxn 1, 2, and 3 of the appropriate construct
Primers: A and B for both reactions
PCR purified and ran 4 ul on a 1% agarose gel
- Ran 4 ul of PCR reaction on a 1% agarose gel