Team:Harvard/color/notebook
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notebook
Week 6 | - | - | - | - | 07-23-2010 |
Week 7 | 07-26-2010 | 07-27-2010 | 07-28-2010 | 07-29-2010 | 07-30-2010 |
07-23-2010 [top]
- Received primers for LUT2 and BETA-OHASE 1 - began building knockdown constructs
- PCR reaction on RS300 to insert recognition sites. Primers listed below
- LUT2 rxn 1: A, CP4
- LUT2 rxn 2: CP2, CP3
- LUT2 rxn 3: CP1, B
- BETA-OHASE 1 rxn 1: A, CP8
- BETA-OHASE 1 rxn 2: CP6, CP7
- BETA-OHASE 1 rxn 3: CP5, B
- PCR purified finished reaction and ran 4 ul on a 1% agarose gel
- Cut out backbone (top) bands from O1, O2, R1 and saved them at 4°C