Team:UTDallas/Protocols
Protocols
Preparing Agar Plates Protocol (Makes 12 (15mm) Plates)
- 300 mL DI H2O + 11 g LB agar
- Autoclave
- Put control thermometer in H2O (from the sink)
- Select vented container mode (Do Not Change Program)
- Mix well after autoclaving; let cool to 50oC
- Add antibiotic (50 to 100 µg/mL) (15 mg total)
- Weigh on paper
- Add to 0.5 mL DI H2O
- Add to LB mixture when cool enough
- Plate
- Under flame open lids of all plates
- Slowly pour agar into plate, avoiding bubbles, when it touches all edges stop pouring
- Let sit under flames until gel solidifies
- Replace lids on plates
- Store upside down at 40oC