Team:Newcastle/23 June 2010

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Contents 1 Wednesday Morning 1.1 Aims 1.2 Materials 1.3 Transformation 2 Wednesday afternoon - taking a break at the Hoppings!!! 2.1 Aims 2.2 Materials

Wednesday Morning

Aims

The aim of today's Lab practice was to transform competent cells with GFP and RFP inserts in an RFP backbone.

Materials

• E.coli broth culture
• Pipettes
• Plasmid ligation products from yesterday
• Conical flasks

Transformation

Cells are made competent ready for transformation.

• Positive control(plasmid uncut)- to make sure cells are transfroming properly
• Negative control (just cells)- nothing but competent cells

5 microfuge tubes:

• 1:5
• 1:3
• Vector
• Negative add nothing
• Positive add 1 μl uncut

Wednesday afternoon - taking a break at the Hoppings!!!

Aims

The aim of this Lab practice was to have lots of fun!

Materials

• Candy floss
• Suntan lotion
• Teddy bears
• Burgers and bacon
• Chips and gravy
• Icecream
• Meerkat toys
• Madmouse ride!
• Beer, wine, lemonade
• Football

	Call to arms!	Philamentous!!!