Team:Newcastle/23 June 2010

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Contents 1 Wednesday Morning 1.1 Aims 1.2 Equipment List 1.3 Transformation 2 Wednesday afternoon taking a break 2.1 Aims 2.2 Equipment list

Wednesday Morning

Aims

The aim of today's Lab practice was to transform competent cells with GFP and RFP inserts in an RFP backbone.

Equipment List

• E.coli broth culture
• Pipettes
• Plasmid ligation products from yesterday
• Conical flasks

Transformation

Cells are made competent ready for transformation.

• Positive control(plasmid uncut)- to make sure cells are transfroming properly
• Negative control (just cells)- nothing but competent cells

5 microfuge tubes:

• 1:5
• 1:3
• Vector
• Negative add nothing
• Positive add 1μl uncut

Wednesday afternoon taking a break

Aims

The aim of this Lab practice was to have fun.

Equipment list

• Candy floss
• Suntan lotion
• Teddy bears
• Burgers and bacon
• Chips and gravy
• Icecream
• Meerkat toys
• Madmouse ride!
• Beer, wine, lemonade
• Football

	Call to arms!	Philamentous