"
Team:Newcastle/Gel electrophoresis
From 2010.igem.org
Revision as of 09:41, 27 July 2010 by RachelBoyd (Talk | contribs)
| |||||||||||||
| |||||||||||||
Gel electrophoresis
- Transfer harden gel into the gel tank
- add TAE buffer until the gel is completely submerged
- Depending on the nature of the sample, 3μl of GeneRuler™ 1kb Plus DNA Ladder
- Loading buffer was then added together with the sample before loading onto the gel matrix
- Run gel at 90V until separation is achieved and visualize using the gelDoc
