Team:Cambridge/References/ProjectBioluminescence/Experiments
From 2010.igem.org
![E. glowli](https://static.igem.org/mediawiki/2010/d/d4/CamBillHeaderSq.png)
Firefly
1. Test luminescence with the Luciferase from the registry
- Transform WT E.coli with the luciferase from the registry.
- Feed with D-luciferin, develop a method for measuring light output
- Variables - concentration of inducer (if luciferase promoter inducible), concentration of D-luciferin
- Controls - no luciferin, no inducer
2. Test use of CHBT as a substrate
a) D-cysteine With sucessfully transformed cells from 1 compare emission when adding:
- D-luciferin
- CHBT
- CHBT + D-Cysteine
- CHBT + L-Cysteine
- (CHBT + L-Luciferin? if necessary)
b) L-cysteine via L-luciferin