Firefly

1. Test luminescence with the Luciferase from the registry

• Transform WT E.coli with the luciferase from the registry.
• Feed with D-luciferin, develop a method for measuring light output
• Variables - concentration of inducer (if luciferase promoter inducible), concentration of D-luciferin
• Controls - no luciferin, no inducer

2. Test use of CHBT as a substrate

a) D-cysteine With sucessfully transformed cells from 1 compare emission when adding:

• • D-luciferin
  • CHBT
  • CHBT + D-Cysteine
  • CHBT + L-Cysteine
  • (CHBT + L-Luciferin? if necessary)

b) L-cysteine via L-luciferin