Team:Calgary/22 July 2010

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Revision as of 16:40, 23 July 2010 by Cktang (Talk | contribs)

Notebook

May
MTWTFSS
          [http://2010.igem.org/Team:Calgary/1_May_2010 1] [http://2010.igem.org/Team:Calgary/2_May_2010 2]
[http://2010.igem.org/Team:Calgary/3_May_2010 3] [http://2010.igem.org/Team:Calgary/4_May_2010 4] [http://2010.igem.org/Team:Calgary/5_May_2010 5] [http://2010.igem.org/Team:Calgary/6_May_2010 6] [http://2010.igem.org/Team:Calgary/7_May_2010 7] [http://2010.igem.org/Team:Calgary/8_May_2010 8] [http://2010.igem.org/Team:Calgary/9_May_2010 9]
[http://2010.igem.org/Team:Calgary/10_May_2010 10] [http://2010.igem.org/Team:Calgary/11_May_2010 11] [http://2010.igem.org/Team:Calgary/12_May_2010 12] [http://2010.igem.org/Team:Calgary/13_May_2010 13] [http://2010.igem.org/Team:Calgary/14_May_2010 14] [http://2010.igem.org/Team:Calgary/15_May_2010 15] [http://2010.igem.org/Team:Calgary/16_May_2010 16]
[http://2010.igem.org/Team:Calgary/17_May_2010 17] [http://2010.igem.org/Team:Calgary/18_May_2010 18] [http://2010.igem.org/Team:Calgary/19_May_2010 19] [http://2010.igem.org/Team:Calgary/20_May_2010 20] [http://2010.igem.org/Team:Calgary/21_May_2010 21] [http://2010.igem.org/Team:Calgary/22_May_2010 22] [http://2010.igem.org/Team:Calgary/23_May_2010 23]
[http://2010.igem.org/Team:Calgary/24_May_2010 24] [http://2010.igem.org/Team:Calgary/25_May_2010 25] [http://2010.igem.org/Team:Calgary/26_May_2010 26] [http://2010.igem.org/Team:Calgary/27_May_2010 27] [http://2010.igem.org/Team:Calgary/28_May_2010 28] [http://2010.igem.org/Team:Calgary/29_May_2010 29] [http://2010.igem.org/Team:Calgary/30_May_2010 30]
[http://2010.igem.org/Team:Calgary/31_May_2010 31]

June
MTWTFSS
  [http://2010.igem.org/Team:Calgary/1_June_2010 1] [http://2010.igem.org/Team:Calgary/2_June_2010 2] [http://2010.igem.org/Team:Calgary/3_June_2010 3] [http://2010.igem.org/Team:Calgary/4_June_2010 4] [http://2010.igem.org/Team:Calgary/5_June_2010 5] [http://2010.igem.org/Team:Calgary/6_June_2010 6]
[http://2010.igem.org/Team:Calgary/7_June_2010 7] [http://2010.igem.org/Team:Calgary/8_June_2010 8] [http://2010.igem.org/Team:Calgary/9_June_2010 9] [http://2010.igem.org/Team:Calgary/10_June_2010 10] [http://2010.igem.org/Team:Calgary/11_June_2010 11] [http://2010.igem.org/Team:Calgary/12_June_2010 12] [http://2010.igem.org/Team:Calgary/13_June_2010 13]
[http://2010.igem.org/Team:Calgary/14_June_2010 14] [http://2010.igem.org/Team:Calgary/15_June_2010 15] [http://2010.igem.org/Team:Calgary/16_June_2010 16] [http://2010.igem.org/Team:Calgary/17_June_2010 17] [http://2010.igem.org/Team:Calgary/18_June_2010 18] [http://2010.igem.org/Team:Calgary/19_June_2010 19] [http://2010.igem.org/Team:Calgary/20_June_2010 20]
[http://2010.igem.org/Team:Calgary/21_June_2010 21] [http://2010.igem.org/Team:Calgary/22_June_2010 22] [http://2010.igem.org/Team:Calgary/23_June_2010 23] [http://2010.igem.org/Team:Calgary/24_June_2010 24] [http://2010.igem.org/Team:Calgary/25_June_2010 25] [http://2010.igem.org/Team:Calgary/26_June_2010 26] [http://2010.igem.org/Team:Calgary/27_June_2010 27]
[http://2010.igem.org/Team:Calgary/28_June_2010 28] [http://2010.igem.org/Team:Calgary/29_June_2010 29] [http://2010.igem.org/Team:Calgary/30_June_2010 30]

July
MTWTFSS
      [http://2010.igem.org/Team:Calgary/1_July_2010 1] [http://2010.igem.org/Team:Calgary/2_July_2010 2] [http://2010.igem.org/Team:Calgary/3_July_2010 3] [http://2010.igem.org/Team:Calgary/4_July_2010 4]
[http://2010.igem.org/Team:Calgary/5_July_2010 5] [http://2010.igem.org/Team:Calgary/6_July_2010 6] [http://2010.igem.org/Team:Calgary/7_July_2010 7] [http://2010.igem.org/Team:Calgary/8_July_2010 8] [http://2010.igem.org/Team:Calgary/9_July_2010 9] [http://2010.igem.org/Team:Calgary/10_July_2010 10] [http://2010.igem.org/Team:Calgary/11_July_2010 11]
[http://2010.igem.org/Team:Calgary/12_July_2010 12] [http://2010.igem.org/Team:Calgary/13_July_2010 13] [http://2010.igem.org/Team:Calgary/14_July_2010 14] [http://2010.igem.org/Team:Calgary/15_July_2010 15] [http://2010.igem.org/Team:Calgary/16_July_2010 16] [http://2010.igem.org/Team:Calgary/17_July_2010 17] [http://2010.igem.org/Team:Calgary/18_July_2010 18]
[http://2010.igem.org/Team:Calgary/19_July_2010 19] [http://2010.igem.org/Team:Calgary/20_July_2010 20] [http://2010.igem.org/Team:Calgary/21_July_2010 21] [http://2010.igem.org/Team:Calgary/22_July_2010 22] [http://2010.igem.org/Team:Calgary/23_July_2010 23] [http://2010.igem.org/Team:Calgary/24_July_2010 24] [http://2010.igem.org/Team:Calgary/25_July_2010 25]
[http://2010.igem.org/Team:Calgary/26_July_2010 26] [http://2010.igem.org/Team:Calgary/27_July_2010 27] [http://2010.igem.org/Team:Calgary/28_July_2010 28] [http://2010.igem.org/Team:Calgary/29_July_2010 29] [http://2010.igem.org/Team:Calgary/30_July_2010 30] [http://2010.igem.org/Team:Calgary/31_July_2010 31]

August
MTWTFSS
            [http://2010.igem.org/Team:Calgary/1_August_2010 1]
[http://2010.igem.org/Team:Calgary/2_August_2010 2] [http://2010.igem.org/Team:Calgary/3_August_2010 3] [http://2010.igem.org/Team:Calgary/4_August_2010 4] [http://2010.igem.org/Team:Calgary/5_August_2010 5] [http://2010.igem.org/Team:Calgary/6_August_2010 6] [http://2010.igem.org/Team:Calgary/7_August_2010 7] [http://2010.igem.org/Team:Calgary/8_August_2010 8]
[http://2010.igem.org/Team:Calgary/9_August_2010 9] [http://2010.igem.org/Team:Calgary/10_August_2010 10] [http://2010.igem.org/Team:Calgary/11_August_2010 11] [http://2010.igem.org/Team:Calgary/12_August_2010 12] [http://2010.igem.org/Team:Calgary/13_August_2010 13] [http://2010.igem.org/Team:Calgary/14_August_2010 14] [http://2010.igem.org/Team:Calgary/15_August_2010 15]
[http://2010.igem.org/Team:Calgary/16_August_2010 16] [http://2010.igem.org/Team:Calgary/17_August_2010 17] [http://2010.igem.org/Team:Calgary/18_August_2010 18] [http://2010.igem.org/Team:Calgary/19_August_2010 19] [http://2010.igem.org/Team:Calgary/20_August_2010 20] [http://2010.igem.org/Team:Calgary/21_August_2010 21] [http://2010.igem.org/Team:Calgary/22_August_2010 22]
[http://2010.igem.org/Team:Calgary/23_August_2010 23] [http://2010.igem.org/Team:Calgary/24_August_2010 24] [http://2010.igem.org/Team:Calgary/25_August_2010 25] [http://2010.igem.org/Team:Calgary/26_August_2010 26] [http://2010.igem.org/Team:Calgary/27_August_2010 27] [http://2010.igem.org/Team:Calgary/28_August_2010 28] [http://2010.igem.org/Team:Calgary/29_August_2010 29]
[http://2010.igem.org/Team:Calgary/30_August_2010 30] [http://2010.igem.org/Team:Calgary/31_August_2010 31]

September
MTWTFSS
    [http://2010.igem.org/Team:Calgary/1_September_2010 1] [http://2010.igem.org/Team:Calgary/2_September_2010 2] [http://2010.igem.org/Team:Calgary/3_September_2010 3] [http://2010.igem.org/Team:Calgary/4_September_2010 4] [http://2010.igem.org/Team:Calgary/5_September_2010 5]
[http://2010.igem.org/Team:Calgary/6_September_2010 6] [http://2010.igem.org/Team:Calgary/7_September_2010 7] [http://2010.igem.org/Team:Calgary/8_September_2010 8] [http://2010.igem.org/Team:Calgary/9_September_2010 9] [http://2010.igem.org/Team:Calgary/10_September_2010 10] [http://2010.igem.org/Team:Calgary/11_September_2010 11] [http://2010.igem.org/Team:Calgary/12_September_2010 12]
[http://2010.igem.org/Team:Calgary/13_September_2010 13] [http://2010.igem.org/Team:Calgary/14_September_2010 14] [http://2010.igem.org/Team:Calgary/15_September_2010 15] [http://2010.igem.org/Team:Calgary/16_September_2010 16] [http://2010.igem.org/Team:Calgary/17_September_2010 17] [http://2010.igem.org/Team:Calgary/18_September_2010 18] [http://2010.igem.org/Team:Calgary/19_September_2010 19]
[http://2010.igem.org/Team:Calgary/20_September_2010 20] [http://2010.igem.org/Team:Calgary/21_September_2010 21] [http://2010.igem.org/Team:Calgary/22_September_2010 22] [http://2010.igem.org/Team:Calgary/23_September_2010 23] [http://2010.igem.org/Team:Calgary/24_September_2010 24] [http://2010.igem.org/Team:Calgary/25_September_2010 25] [http://2010.igem.org/Team:Calgary/26_September_2010 26]
[http://2010.igem.org/Team:Calgary/27_September_2010 27] [http://2010.igem.org/Team:Calgary/28_September_2010 28] [http://2010.igem.org/Team:Calgary/29_September_2010 29] [http://2010.igem.org/Team:Calgary/30_September_2010 30]

October
MTWTFSS
        [http://2010.igem.org/Team:Calgary/1_October_2010 1] [http://2010.igem.org/Team:Calgary/2_October_2010 2] [http://2010.igem.org/Team:Calgary/3_October_2010 3]
[http://2010.igem.org/Team:Calgary/4_October_2010 4] [http://2010.igem.org/Team:Calgary/5_October_2010 5] [http://2010.igem.org/Team:Calgary/6_October_2010 6] [http://2010.igem.org/Team:Calgary/7_October_2010 7] [http://2010.igem.org/Team:Calgary/8_October_2010 8] [http://2010.igem.org/Team:Calgary/9_October_2010 9] [http://2010.igem.org/Team:Calgary/10_October_2010 10]
[http://2010.igem.org/Team:Calgary/11_October_2010 11] [http://2010.igem.org/Team:Calgary/12_October_2010 12] [http://2010.igem.org/Team:Calgary/13_October_2010 13] [http://2010.igem.org/Team:Calgary/14_October_2010 14] [http://2010.igem.org/Team:Calgary/15_October_2010 15] [http://2010.igem.org/Team:Calgary/16_October_2010 16] [http://2010.igem.org/Team:Calgary/17_October_2010 17]
[http://2010.igem.org/Team:Calgary/18_October_2010 18] [http://2010.igem.org/Team:Calgary/19_October_2010 19] [http://2010.igem.org/Team:Calgary/20_October_2010 20] [http://2010.igem.org/Team:Calgary/21_October_2010 21] [http://2010.igem.org/Team:Calgary/22_October_2010 22] [http://2010.igem.org/Team:Calgary/23_October_2010 23] [http://2010.igem.org/Team:Calgary/24_October_2010 24]
[http://2010.igem.org/Team:Calgary/25_October_2010 25] [http://2010.igem.org/Team:Calgary/26_October_2010 26] [http://2010.igem.org/Team:Calgary/27_October_2010 27] [http://2010.igem.org/Team:Calgary/28_October_2010 28] [http://2010.igem.org/Team:Calgary/29_October_2010 29] [http://2010.igem.org/Team:Calgary/30_October_2010 30] [http://2010.igem.org/Team:Calgary/31_October_2010 31]

Thursday July 22, 2010

Himika

Today I miniprepped 10 colonies from the I0500-B0034 construction using Sigma-Aldrich kit. I also did a plasmid switch of I0500-B0034 from pSB1A3 to pSB1AC3.

Jeremy

Today I did a colony PCR of K23900+I13507, K239000+I13504, K135000+I13507, K135000+I13504 and prepared an overnight of several colonies. I also re-ligated and transformed I0500+B0034 in pSB1AC3 plasmid.

Chris

Today, I calculated the values of the enzymes we received from New England Biolabs. As well, I redid the gel of the colony PCR of the CpxP promoter with a 1.0% gel. This time, the DNA actually moved and the picture of the gel can be seen on the right. However, the bands were very non-specific. To optimize, I set up one gradient PCR with temperature of annealing step ranging from 53-58°C.

No notebook page exists for this date. Sorry!

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