Team:Cambridge/References/ProjectBioluminescence/Luciferase

Mutant (EPIC) luciferase (P. pyralis)

• [http://www.ncbi.nlm.nih.gov/nuccore/AB261988.1 Mutant (EPIC) Luciferase (P.pyralis) Sequence]
• [http://www.ncbi.nlm.nih.gov/pubmed/10529195 Site-directed mutagenesis of firefly luciferase active site amino acids: a proposed model for bioluminescence color]
• [http://www.ncbi.nlm.nih.gov/pubmed/12044905 Improved practical usefulness of firefly luciferase by gene chimerization and random mutagenesis]

Bacterial Bioluminescence

• Vibrio Fischeri
• Vibrio Harveyi
• Genetics of luxCDABE (adapted from wikipedia)
• [http://parts.mit.edu/wiki/index.php/Lux_operon| 2006 attempts at BioBricking the lux operon]
• Notes
• [http://partsregistry.org/Lux The lux wiki from parts registry]
• [http://partsregistry.org/wiki/index.php/Part:BBa_I712019 The Firefly Luciferase in the registry]. There is no promotor added onto it presumed by the phrase "acts as a reporter protein when connected to other proteins or promoters"
• [http://partsregistry.org/wiki/index.php/Part:BBa_I712019 The Firefly Luciferase in the registry]. There is no promotor added onto it presumed by the phrase "acts as a reporter protein when connected to other proteins or promoters"

• [http://www.ncbi.nlm.nih.gov/nuccore/AF170104.1?report=graph&log$=seqview NCBI Lux operon sequence]
• [http://partsregistry.org/wiki/index.php/Part:BBa_I712052 Parts Registry Luciferase under control of Phage promoter] - T7 Phage
• IMPORTANT INFO

Plasmid experiment

• [http://departments.kings.edu/biology/lux/bacterial.html Vibrio plasmid experiment] -possible source of lux operon

File:4449975.pdf

• [http://departments.kings.edu/biology/lux/index.html] - Transformation protocol for students from basics - includes several plasmids

• Plan
  • set plasmid 555 into a BioBrick
  • replace the promoter? sensitivity tuners?