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Team:Cambridge/References/ProjectBioluminescence/Luciferase
From 2010.igem.org
Photinus Pyralis (Firefly Luciferase)
EPIC luciferase
- [http://www.ncbi.nlm.nih.gov/nuccore/AB261988.1 Mutant (EPIC) Luciferase (P.pyralis) Sequence]
Bacterial Bioluminescence
- Vibrio Fischeri
- Vibrio Harveyi
- Genetics of luxCDABE (adapted from wikipedia)
- [http://parts.mit.edu/wiki/index.php/Lux_operon| 2006 attempts at BioBricking the lux operon]
- Notes
- [http://partsregistry.org/Lux The lux wiki from parts registry]
- [http://www.ncbi.nlm.nih.gov/nuccore/AF170104.1?report=graph&log$=seqview NCBI Lux operon sequence]
- [http://partsregistry.org/Part:BBa_G10001 parts registry lux operon] -currently waiting on an email to see how useful/available this part is
- IMPORTANT INFO
Plasmid experiment
- [http://departments.kings.edu/biology/lux/bacterial.html Vibrio plasmid experiment] -possible source of lux operon
- [http://departments.kings.edu/biology/lux/index.html] - Transformation protocol for students from basics - includes several plasmids
- Plan
- set plasmid 555 into a BioBrick
- replace the promoter? sensitivity tuners?
