Bioluminescence

The Plan

• Characterise the transfer function of PoPs + CHBT --> Light

The Theory

• The Luciferin Cycle:

Increasing Light Emission

• [http://www.ncbi.nlm.nih.gov/pubmed/12234677 Paper on isolation of G-LRE and H-LRE, which lists previous attempts to improve light production]
• [http://www.ncbi.nlm.nih.gov/pubmed/10036167 Intensified light emission in presence of low ATP concentration by PPDK]
• [http://www.ncbi.nlm.nih.gov/pubmed/1332531 Enhanced luciferase activity through cytodine nucleotides]
• [http://www.ncbi.nlm.nih.gov/pubmed/12044905 Gene chimerisation to improve practical usefulness of firefly luciferase]
• [http://www.ncbi.nlm.nih.gov/pubmed/17540326 Genetically modified firefly luciferase - the EPIC luciferase]

Sequences

• [http://www.ncbi.nlm.nih.gov/nucleotide/14331151?report=genbank&log$=nucltop&blast_rank=1&RID=4EUAXMJW01R A-LRE (P.pyralis) Sequence]
• [http://www.ncbi.nlm.nih.gov/nuccore/AB261988.1 Mutant (EPIC) Luciferase (P.pyralis) Sequence]