Bioluminescence

The Plan

• Characterise the transfer function of PoPs + CHBT --> Light

The Theory

• The Luciferin Cycle:

Increasing Light Emission

• [http://www.ncbi.nlm.nih.gov/pubmed Paper on isolation of H-LRE and G-LRE]

Previous attempts to improve light production of luciferase reaction Pyruvate orthophosphate dikinase (PPDK) converts AMP + PPi to ATP (AMP + PPi + Phosphoenol Pyruvate -> ATP + Pyruvate + Phosphate) => light emission intensified in presence of low ATP concentration

• [http://www.ncbi.nlm.nih.gov/pubmed/1332531 Enhanced luciferase activity through cytodine nucleotides]
• [http://www.ncbi.nlm.nih.gov/pubmed/12044905 Gene chimerisation to improve practical usefulness of firefly luciferase]
• [http://www.ncbi.nlm.nih.gov/pubmed/17540326 Genetically modified firefly luciferase - the EPIC luciferase]

Sequences

• [http://www.ncbi.nlm.nih.gov/nucleotide/14331151?report=genbank&log$=nucltop&blast_rank=1&RID=4EUAXMJW01R A-LRE (P.pyralis) Sequence]
• [http://www.ncbi.nlm.nih.gov/nuccore/AB261988.1 Mutant Luciferase (P.pyralis) Sequence]

Bacterial stuff

[http://departments.kings.edu/biology/lux/bacterial.html Vibrio plasmid]