Monday July 19, 2010

Emily

• Today I aliquoted our BBK-CP-F and BBK-CP-R primers. I then set up and ran a PCR with Taq Polymerase in order to test that our BBK construction primers are working. I also played around with the plate reader with our Arabinose induced cultures of I0500-B0034-E0040 from the weekend. Unfortunately, we didn't see any significant difference in GFP output between our cultures and a control of LB broth. We will start overnight cultures to retry the Arabinose induction procedure tonight. I also started overnight cultures of a J13002-E0040 construct in order to serve as a positive control in our GFP plate reading which we will be trying again tomorrow. Finally, I started a plasmid switch of our four constructs of I0500-B0034 in order to get them into a psb1AK3 vector and I also strated overnight cultures of B0034 in the psb1A2 plasmid, in order to start a plasmid switch of that construct tomorrow.

Patrick

Today, I set up and reupdated the team blog and Twitter. As well, I looked into papers regarding plasmid copy number and some basic JavaScript/CSS code to start constructing portions of the wiki.