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Team:Stanford/NotebookPage/19 July 2010
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Revision as of 20:44, 19 July 2010 by Karpadillo (Talk | contribs)
Laura's Lab notebook
set up the following ligations from the gel extractions done by me, Karina, and Francisco on Friday, 7/14/10)
- Francisco ran and imaged diagnostic gel on Friday
| Ligation Recipe | |
|---|---|
| dH2O | none |
| vector (with terminators attached) | 5.0 uL |
| insert (RFP or GFP) | 12.0 uL |
| 10X buffer | 2.0 uL |
| T4 ligase | 1.0 uL |
- normally run for 10 minutes at room temperature, overnight this time (started at 11:30 am)
