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From 2010.igem.org

Laura's Lab notebook

set up the following ligations from the gel extractions done by me, Karina, and Francisco on Friday, 7/14/10)

• Francisco ran and imaged diagnostic gel on Friday

Ligation Recipe
dH2O	none
vector (with terminators attached)	5.0 uL
insert (RFP or GFP)	12.0 uL
10X buffer	2.0 uL
T4 ligase	1.0 uL

• normally run for 10 minutes at room temperature, overnight this time (started at 11:30 am)

Nanodrop data (deemed unreliable based on 260/230, possibly due do residual EtBr contamination)

part	260/280	260/230	ng/uL
vector/terminator	1.77	0.03	19.1
RFP	1.86	0.02	16.2
GFP	1.83	0.02	14.8