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From 2010.igem.org

Lab work

Characterization of Anderson RBS sequences

Restriction digestion of plasmid backbone pSB1T3 using Buffer H of Roche.

Digestion reaction: needed fragment

1,0 μg pSB1T3 + 1 U/μL EcoRI and 1 U/μL PstI: ‘E – ---- – P’

The digested pSB1T3 was cut from gel and isolate the band. However, the gel extraction kit didn’t work as well as we had hoped, it turned out to be the DNA disappearing kit. Tomorrow a new day.