Team:Minnesota/Project
From 2010.igem.org
You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing. | |
Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs) | |
Team Example |
Home | The Team | The Project | SynBioSS Designer | Modeling | Experimental | Competition Requirements |
---|
Contents |
Overall project
Metabolic Engineering: In vivo Nanobioreactors
Modern microbial engineering methods allow the introduction of useful exogenous metabolic pathways into cells. Metabolism of certain organic compounds is sometimes limited by the production of toxic intermediates. Several bacteria have evolved protein based microcompartments capable of sequestering such reactions, thus protecting cytosolic machinery and processes from interference by these intermediates. For our project, we will identify and transform the genes encoding proteins responsible for the production and assembly of bacterial microcompartments. Additionally, we will confirm the signal sequences that target enzymes to the protein compartments by fusing this sequence to reporter genes. To demonstrate the microcompartment’s potential to serve as nanobioreactors, we will target genes encoding a short catabolic pathway into recombinant microcompartments assembled in E. coli.