Team:Penn State/Project

From 2010.igem.org

Revision as of 16:09, 16 July 2010 by Erik (Talk | contribs)


Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety



Contents

Overall project

The Penn State Team will be researching and experimenting two different ideas. The first area of study will be creating an oxygen-sensing promoter. This will be done through researching the way that FNR and arcA regulate aerobic/anaerobic respiration in E. coli; engineering applications will be applied to these processes. The second topic we are working on is Bacterial Fireworks, an incorporation of quorum sensing, pigments, and cell lysis. Our goal is to assemble the necessary parts to display the spreading of color as the cells lyse. The team would like to incorporate both aspects of the project to create a bacterial fireworks display that is triggered by a lack of oxygen inside of an agar sphere.


A reliable modular lysis mechanism through quorum sensing could be used in many different fields. As engineered microorganisms are becoming more widely considered for use in the open environment, concerns are growing about possible detrimental effects these microbes could have on ecosystems if they were ever to grow and multiply past expectations. A quorum-based lysis "safety" mechanism would only require one cell to receive a predetermined signal in order to eliminate the entire rogue population, should it escape the area of its intended use.

Project Details

Genetic Circuit 1

The Penn State iGEM team began work and research in mid May. After learning lab techniques from the grad students, we began researching possible topics. We decided to create a cell lysis device.


Initially, we transformed Lux Promoters, Constitutive Promoters, LuxI + RBS, LuxR + RBS, Lambda Phage lysis devices, Terminators, LacI, LuxR, pLac, CI, RBS, Pigments, FP, LacI + RBS, and CI + RBS.

Lux Promoters Lux Promoters Constitutive Promoters LuxI + RBS LuxR + RBS Lambda Phage Lysis Device Terminator
K091107 R0061 J23108 C0261 J37033 K112022 B0010
K091146 R0063 J23116 S01414 K112808 B1002
K145150 R1062 J23100 B1006


LuxR LacI pLac CI RBS Pigment FP LacI + RBS CI + RBS
C0062 C0012 R0010 C0051 B0033 K274100 RFP + RBS J24679 K081007
B0034 K274004 C0012 + B0034 P0451


Our finalized assembly looked like this; however, this would not be the finalization of our project.


Lysis.jpg

The Experiments

Genetic Circuit Assembly

Our second part is a redo of the lysis device.

Lysis2.jpg

Part 3

Results