Team:Heidelberg/Notebook/BSDesign/October
From 2010.igem.org
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Binding Site Design - October02/10/2010
05/10/2010
06/10/201020 Miniprep:
12/10/20104 miraPCRs for binding sites of miR4286.
13/10/2010
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Binding Site Design - October02/10/2010
05/10/2010
06/10/201020 Miniprep:
12/10/20104 miraPCRs for binding sites of miR4286.
13/10/2010
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
all negative
ligation of miMeasure construct:
miR-122
miR-122ran
miR-1179
miR-221
each in 0S/0L/10S/10L versions
transformation of each ligation to TOP10 E.coli
cells were plated on LB-amp plates
96 colony PCRs - 8 from each plate
ALL NEGATIVE!!!!