Team:Heidelberg/Notebook/miMeasure

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miMeasure

miMeasure allows to quantitatively detect endogenous miRNA in different cells. To detect miRNA one should clone binding site for it into 3' UTR of GFP gene. To ensure that transfection efficiency or amount of cells do not affect result we decided to include reference fluorescence protein, BFP to allow normalisation.


miMeasure plasmid

Retrieved from "http://2010.igem.org/Team:Heidelberg/Notebook/miMeasure"