Team:Osaka/week14

From 2010.igem.org

October 24 (Sun)

1. Transformation
   • 10/23 ligation sample
2. miniprep
   • 089Y, 100, 131Y, 039Y, 034, 025, 138, 129
3. Treatment of restriction enzyme
   • 129, 100, 089Y, 131Y, 138, 039
4. M9 culture
5. Electrophoresis
   • 039 x
   • 089Y OK
   • 138 x
   • 100 x
   • 129 x
   • 131Y ok
6. tranformation
   • 91, 93
7. make amorphous cellulose

October 25 (Mon)

1. miniprep
   • 039, 107, 129, 138
2. make Pi buffer
3. amorphous cellulose
4. Electrophoresis
   • 138 x
   • 129 ok
   • 107 ok
   • 039 x
5. transformation
   • 100
6. To liquid culture
   • 100, 132, 132Y, 089Y, 064-2, 101, 129, 035-2, 094, 123, 126, 132, 091-2, 130

October 26 (Tue)

1. miniprep
   • 132, 130, 091-2, 123, 126, 091-1, 035-2, 093-1, 013, 129, 100, 089Y, 131, 064-2, 094, 132-1, 132Y, 100
2. Treatment of restriction enzyme
   • today's miniprep samples
3. Electrophoresis
   • 013 ok
   • 035 x
   • 089Y ok
   • 091 ok
   • 093 ok
   • 100 x
   • 101 x
   • 126 x
   • 123 x
   • 129 ok
   • 130 ok
   • 132Y ?
   • 064-5  ?
   • 093 ok
   • 094 ok
   • 132 ok
   • 132Y ok
4. Ligation
   • 039 + 079
   • 075 + 1-23L
   • 2-4A + 102
   • 130 + BYP
5. measurement OD

October 27 (Wed)

1. miniprep
2. Treatment of restriction enzyme
   • 031, 100X, 100Y, 100Z
3. Electrophoresis
4. measurement cellulase

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