Team:Queens-Canada/23 July 2010

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July 23, 2010

Gel Electrophoresis

Thai

eYFP, pSB1A3

Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 100 V.

PCR

Yuli and Chris

pSB1A3

Program run using 9:1 FAST:HIFI blend with an extension time of 70 s and annealing temperature of 65 °C.

Microinjection

Hao

Practiced injections with water.

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