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Team:Queens-Canada/23 July 2010
From 2010.igem.org
July 23, 2010
Gel Electrophoresis
Thai
eYFP, pSB1A3
Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 100 V.
PCR
Yuli and Chris
pSB1A3
Program run using 9:1 FAST:HIFI blend with an extension time of 70 s and annealing temperature of 65 °C.
Microinjection
Hao
Practiced injections with water.
