Team:Queens-Canada/21 July 2010

Gel Electrophoresis

Thai and Yuli

Used 0.4 g agarose in 50 mL TBE. Gel run for 45 mins at 100 V.

Gel Electrophoresis

Yuli and Thai

Used 0.4 g agarose in 60 mL TBE.

PCR

Hao

pSip-1

Program run using 9:1 FAST:HIFI blend with an extension time of 6 s and annealing temperature of 55 °C.

Gel Extraction

Yuli

eYFP1a

Eluted extract with 30 µL of elution buffer.

Gel Electrophoresis

Chris and Mary

pSip-1

Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 100 V.

Microinjection

Hao Practiced injections with water.