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Team:Queens-Canada/21 July 2010
From 2010.igem.org
July 21, 2010
Gel Electrophoresis
Thai and Yuli
Used 0.4 g agarose in 50 mL TBE. Gel run for 45 mins at 100 V.
Gel Electrophoresis
Yuli and Thai
Used 0.4 g agarose in 60 mL TBE.
PCR
Hao
pSip-1
Program run using 9:1 FAST:HIFI blend with an extension time of 6 s and annealing temperature of 55 °C.
Gel Extraction
Yuli
eYFP1a
Eluted extract with 30 µL of elution buffer.
Gel Electrophoresis
Chris and Mary
pSip-1
Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 100 V.
Microinjection
Hao Practiced injections with water.
