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Team:Queens-Canada/14 July 2010
From 2010.igem.org
July 14, 2010
Digestion
Thai
pSBII
Cut using BamHI.
Gel Electrophoresis
Thai
pBSII
Used 0.5 g agarose in 70 mL TBE. Gel run for 50 mins at 100 V.
Heat Shock
Chris and Geoff
pStr-1, BBa_I13522
Plates stored in 37 °C room overnight.
Diagnostic PCR
Yuli and Hao
pStr-1
Program run using HIFI/FAST blends with extension times of 30 s and annealing temperature of 55 °C. HIFI and FAST buffers were also blended.
| Polymerase | ||||||
|---|---|---|---|---|---|---|
| FAST:HIFI | 9:1 | 4:1 | 1:1 | 1:0 | 0:1 | |
| Buffer | 1:0 | A | B | C | D | E |
| 0:1 | F | G | H | I | X | |
| 1:1 | K | L | M | N | P | |
Gel Electrophoresis
Thai and Mary
pStr-1
Used 0.7 g agarose in 100 mL TBE. Gel run for 40 mins at 100 V.
Microinjection
Hao Practiced injections with water.
