Team:Queens-Canada/14 July 2010

From 2010.igem.org

July 14, 2010

Digestion

Thai

pSBII

Cut using BamHI.

Gel Electrophoresis

Thai

pBSII

Used 0.5 g agarose in 70 mL TBE. Gel run for 50 mins at 100 V.

Heat Shock

Chris and Geoff

pStr-1, BBa_I13522

Plates stored in 37 °C room overnight.

Diagnostic PCR

Yuli and Hao

pStr-1

Program run using HIFI/FAST blends with extension times of 30 s and annealing temperature of 55 °C. HIFI and FAST buffers were also blended.


Polymerase
FAST:HIFI 9:1 4:1 1:1 1:0 0:1
Buffer 1:0 A B C D E
0:1 F G H I X
1:1 K L M N P

Gel Electrophoresis

Thai and Mary

pStr-1

Used 0.7 g agarose in 100 mL TBE. Gel run for 40 mins at 100 V.

Microinjection

Hao Practiced injections with water.