Revision as of 00:21, 28 October 2010 by Yessa (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Contents 1 Transformation of ligated yneA into pGFPrrnB and pSB1C3 1.1 Aims 1.2 Materials and Protocol 2 Plating Bacillus subtilis BFS678

Transformation of ligated yneA into pGFPrrnB and pSB1C3

Aims

To produce more colonies of the plasmid pGFPrrnB and pSB1C3 containing yneA with pGFPrrnB and pSB1C3 fromyesterday.

Materials and Protocol

Please refer to transformation of E. coli.

Plating Bacillus subtilis BFS678

Bacillus subtilis BFS678 has the pMutin4 (and thus lacI) integrated into the chromosome which will be useful for characterisation. Therefore we will be using this strain for transformation with our filamentous cell and arginase BioBricks.