ULB/5 October 2010

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"Biobricks construction"

  • PCR to obtain linearised pSB1C3, using linearised pSB1C3 as template, with the protocol from iGEM parts registry. Didn't work.
  • PCR on ParD and ParE to have those genes outside of a plasmid (as PCR products). Worked. We used a plasmid containing both genes as template.
  • PCR on FLP, using the sequence we got synthesized as backbone, to add RCF 10 prefix and suffix to the gene. Didn't work.

"Module hydrogen"

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